本發明(ming)屬于醫用檢(jian)測(ce)，具體涉(she)及便攜熒光檢(jian)測(ce)器。

背景技術：

1、每年(nian)由于(yu)hbv相關(guan)肝病導致的(de)(de)(de)死亡占(zhan)世界hbv死亡率(lv)的(de)(de)(de)30％，達30萬例。肝臟(zang)(zang)儲備功(gong)(gong)能(neng)反(fan)映(ying)肝臟(zang)(zang)耐受損傷的(de)(de)(de)能(neng)力，肝臟(zang)(zang)儲備功(gong)(gong)能(neng)的(de)(de)(de)全面評估，對判(pan)斷(duan)肝臟(zang)(zang)病情與預后、制定治療方案具(ju)有重要意義。術(shu)前(qian)吲哚菁綠15分(fen)鐘滯留率(lv)試驗是目前(qian)臨床上(shang)引用(yong)最廣泛的(de)(de)(de)定量肝功(gong)(gong)能(neng)評估實(shi)驗。吲哚菁綠肝臟(zang)(zang)儲備功(gong)(gong)能(neng)分(fen)析(xi)系(xi)統是目前(qian)臨床唯一可(ke)以實(shi)現床旁、無創、準確(que)、實(shi)時地(di)反(fan)映(ying)肝臟(zang)(zang)儲備功(gong)(gong)能(neng)的(de)(de)(de)檢測(ce)技(ji)術(shu)，但是該系(xi)統體積大、成本高(gao)。

2、在(zai)(zai)現有技術中，其(qi)他檢測(ce)肝(gan)(gan)儲備(bei)功(gong)能(neng)(neng)的方(fang)法，如(ru)膽紅素，肝(gan)(gan)臟(zang)酶類(lei)指(zhi)標，如(ru)血清谷(gu)草轉(zhuan)(zhuan)氨酶、血清谷(gu)丙轉(zhuan)(zhuan)氨酶，以及child-pugh?分級法等，都存在(zai)(zai)一定(ding)問題：不能(neng)(neng)完全有效評估肝(gan)(gan)癌患者(zhe)肝(gan)(gan)儲備(bei)功(gong)能(neng)(neng)；不具有即(ji)時性，間(jian)隔檢查間(jian)存在(zai)(zai)重(zhong)疊；不利于全面評估肝(gan)(gan)儲備(bei)功(gong)能(neng)(neng)。

3、中國專利?cn103385695多波長肝臟儲(chu)備功能(neng)檢測儀中，雖然該儀器可將參數(shu)存(cun)儲(chu)于sd卡(ka)或數(shu)據存(cun)儲(chu)器中，方便獲取直觀(guan)數(shu)據，但(dan)由于光信號到電信號到數(shu)字信號的過程中，各(ge)肝功能(neng)參數(shu)測量值與(yu)實際值誤差較大，且離真正投入市場仍有(you)一段距離。

技術實現思路

1、本發明的(de)目的(de)在(zai)于提(ti)供便攜熒(ying)光檢測器，實現評估(gu)患者肝(gan)臟儲(chu)備功能，以解決(jue)上(shang)述背(bei)景技術中提(ti)出(chu)的(de)問題。

2、為(wei)實現(xian)上(shang)述目的，本發(fa)明提供(gong)如(ru)下技術方(fang)案(an)：便攜熒光(guang)檢測器，該檢測器包括供(gong)電(dian)(dian)電(dian)(dian)路，激發(fa)光(guang)源，采樣(yang)調理(li)，信(xin)號處理(li)、光(guang)電(dian)(dian)接收器、微處理(li)器、數(shu)據存儲電(dian)(dian)路；

3、所述激發光(guang)源與(yu)供電(dian)電(dian)路相連，而光(guang)電(dian)接收器(qi)與(yu)高精度ua級電(dian)流檢測電(dian)路相連；所述光(guang)電(dian)接收器(qi)用于接受穿過(guo)人體或者動物組織的(de)熒光(guang)發射光(guang)并將(jiang)其轉換成電(dian)信號；

4、其中：

5、所(suo)述(shu)采(cai)樣(yang)調理部分由(you)高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)和(he)高(gao)(gao)(gao)(gao)精(jing)度ua級電(dian)(dian)(dian)(dian)(dian)流檢測(ce)電(dian)(dian)(dian)(dian)(dian)路組成(cheng)(cheng)，所(suo)述(shu)高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)優(you)選(xuan)(xuan)(xuan)為(wei)750nm、800nm、825nm高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)，更(geng)優(you)選(xuan)(xuan)(xuan)為(wei)780nm高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)，最優(you)選(xuan)(xuan)(xuan)為(wei)850nm高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)；所(suo)述(shu)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)電(dian)(dian)(dian)(dian)(dian)接(jie)(jie)收器(qi)(qi)與(yu)高(gao)(gao)(gao)(gao)精(jing)度ua級電(dian)(dian)(dian)(dian)(dian)流檢測(ce)電(dian)(dian)(dian)(dian)(dian)路連接(jie)(jie)，當激(ji)發(fa)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)源發(fa)射出優(you)選(xuan)(xuan)(xuan)的(de)(de)(de)660nm～780nm波(bo)(bo)長的(de)(de)(de)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)源，照(zhao)射至含有熒光(guang)(guang)(guang)(guang)(guang)(guang)(guang)染料的(de)(de)(de)液體或者(zhe)組織，產生熒光(guang)(guang)(guang)(guang)(guang)(guang)(guang)，高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)過(guo)濾掉燈珠(zhu)波(bo)(bo)長及(ji)自(zi)然(ran)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)，透過(guo)熒光(guang)(guang)(guang)(guang)(guang)(guang)(guang)染料被燈珠(zhu)激(ji)發(fa)的(de)(de)(de)發(fa)射光(guang)(guang)(guang)(guang)(guang)(guang)(guang)，光(guang)(guang)(guang)(guang)(guang)(guang)(guang)電(dian)(dian)(dian)(dian)(dian)接(jie)(jie)收器(qi)(qi)接(jie)(jie)收透過(guo)優(you)選(xuan)(xuan)(xuan)為(wei)850nm高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)的(de)(de)(de)發(fa)射光(guang)(guang)(guang)(guang)(guang)(guang)(guang)，將(jiang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)信號(hao)轉(zhuan)換(huan)成(cheng)(cheng)微(wei)電(dian)(dian)(dian)(dian)(dian)流，且在850nm高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)波(bo)(bo)長附近的(de)(de)(de)相對感光(guang)(guang)(guang)(guang)(guang)(guang)(guang)靈敏(min)度最高(gao)(gao)(gao)(gao)，低于該(gai)高(gao)(gao)(gao)(gao)通(tong)(tong)(tong)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)波(bo)(bo)長或者(zhe)高(gao)(gao)(gao)(gao)于該(gai)濾光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)波(bo)(bo)長的(de)(de)(de)感光(guang)(guang)(guang)(guang)(guang)(guang)(guang)靈敏(min)度逐漸降低，而高(gao)(gao)(gao)(gao)精(jing)度ua級電(dian)(dian)(dian)(dian)(dian)流檢測(ce)電(dian)(dian)(dian)(dian)(dian)路將(jiang)接(jie)(jie)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)電(dian)(dian)(dian)(dian)(dian)收器(qi)(qi)產生的(de)(de)(de)微(wei)電(dian)(dian)(dian)(dian)(dian)流通(tong)(tong)(tong)過(guo)電(dian)(dian)(dian)(dian)(dian)阻轉(zhuan)換(huan)為(wei)電(dian)(dian)(dian)(dian)(dian)壓(ya)(ya)，再經過(guo)放大和(he)濾波(bo)(bo)后得(de)到(dao)放大后的(de)(de)(de)輸出電(dian)(dian)(dian)(dian)(dian)壓(ya)(ya)，轉(zhuan)化(hua)成(cheng)(cheng)電(dian)(dian)(dian)(dian)(dian)壓(ya)(ya)信號(hao)由(you)單(dan)片(pian)機adc讀取(qu)，單(dan)片(pian)機adc斷電(dian)(dian)(dian)(dian)(dian)時，數據(ju)(ju)(ju)將(jiang)存(cun)儲(chu)至數據(ju)(ju)(ju)存(cun)儲(chu)器(qi)(qi)，通(tong)(tong)(tong)過(guo)裝置轉(zhuan)換(huan)器(qi)(qi)讀取(qu)數據(ju)(ju)(ju)到(dao)pc端上位(wei)機，進行數據(ju)(ju)(ju)統計及(ji)分析，軟件窗口能夠以txt文本的(de)(de)(de)形式傳輸出來(lai)數據(ju)(ju)(ju)；

6、所述(shu)信號處(chu)理部(bu)分由數(shu)(shu)據存儲(chu)器和(he)上位機讀取(qu)組成，所述(shu)上位機通過軟件讀取(qu)經(jing)過高精度(du)ua級電(dian)流檢測(ce)電(dian)路處(chu)理后的電(dian)壓(ya)值，并(bing)轉(zhuan)化成光強參(can)數(shu)(shu)。

7、優選的(de)，所述供電(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)路由充(chong)(chong)(chong)電(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)路和(he)線性穩(wen)壓電(dian)(dian)(dian)(dian)路構成(cheng)；充(chong)(chong)(chong)電(dian)(dian)(dian)(dian)電(dian)(dian)(dian)(dian)路當插(cha)上(shang)裝(zhuang)置轉換器時立即開(kai)始工(gong)作，對鋰電(dian)(dian)(dian)(dian)池進行充(chong)(chong)(chong)電(dian)(dian)(dian)(dian)；線性穩(wen)壓電(dian)(dian)(dian)(dian)路將鋰電(dian)(dian)(dian)(dian)池電(dian)(dian)(dian)(dian)壓轉成(cheng)穩(wen)定的(de)3.3v電(dian)(dian)(dian)(dian)壓輸出給后面負載設備供電(dian)(dian)(dian)(dian)。

8、優選的，所(suo)(suo)述激發光(guang)源(yuan)部(bu)分由(you)開(kai)(kai)關控制電路和燈(deng)珠，所(suo)(suo)述燈(deng)珠范(fan)圍值(zhi)優選為700nm～780、720nm～780nm、740～780nm，再優選為660nm～780nm、更優選為680～780nm組成；通(tong)過(guo)開(kai)(kai)關控制供電電路實現對燈(deng)珠持續性發光(guang)和間斷性發光(guang)兩(liang)種模式的切換；更優選燈(deng)珠由(you)線性穩(wen)壓(ya)電路穩(wen)定供電，發射出波(bo)長范(fan)圍為630nm～730nm，中心(xin)波(bo)長為665nm，隨著距離增加波(bo)長范(fan)圍至(zhi)597nm～992nm。

9、優選的(de)，所(suo)述(shu)裝置轉換器為ttl串usb模塊，用于(yu)將電(dian)路板儲存的(de)數據通過usb傳輸至電(dian)腦(nao)。

10、優選(xuan)的(de)，該檢測器的(de)使(shi)用步驟如下：

11、步(bu)驟一：靜脈注射(she)熒光(guang)染料

12、步(bu)驟二：連接(jie)電源(yuan)，將(jiang)裝置(zhi)貼在(zai)需檢測的部位，進(jin)行數據采集

13、步驟(zou)三(san)：采集結束，取下裝置(zhi)

14、步驟四：裝置連接電(dian)路，顯示參(can)數(shu)，保存數(shu)據

15、步驟五：是(shi)否清除數據(ju)或(huo)繼(ji)續采集

16、步驟六(liu)：斷開電源，結束(shu)。

17、優選的，icg色素15分鐘滯留(liu)率的計算公式如(ru)下：

18、

19、其(qi)中(zhong)，t為時間，b由線(xian)性回歸方程求得；

20、

21、cg是和r15相關的(de)濃度系(xi)數(shu)，為常數(shu)項(xiang)，為t時刻的(de)icg色(se)素(su)值，為t=0時注射icg的(de)色(se)素(su)值；采用最小二乘法，線性回歸分析球的(de)數(shu)學中(zhong)的(de)系(xi)數(shu)項(xiang)與的(de)值。

22、與現有(you)技(ji)術(shu)相比，本發(fa)明的(de)有(you)益效果是(shi)：

23、1、本發明(ming)裝置體積小(xiao)，續航時(shi)間長(chang)達48小(xiao)時(shi)且可按需求增加至(zhi)更久，電池(chi)可于使(shi)用中替(ti)換，便(bian)于隨身佩戴，檢測的(de)同(tong)時(shi)不影響(xiang)患者活動；

24、2、發明裝置的(de)存(cun)(cun)儲(chu)電(dian)(dian)路能儲(chu)存(cun)(cun)掉電(dian)(dian)時的(de)數據(ju)，即使在斷電(dian)(dian)的(de)情況下，也能將數據(ju)儲(chu)存(cun)(cun)到(dao)片外存(cun)(cun)儲(chu)，再次(ci)上電(dian)(dian)時仍能識別情況進行讀(du)取操(cao)作，當存(cun)(cun)儲(chu)頻(pin)率(lv)(lv)為5秒/次(ci)時，可以連續存(cun)(cun)儲(chu)18h數據(ju)，且可根據(ju)需求調整存(cun)(cun)儲(chu)頻(pin)率(lv)(lv)；

25、3、實(shi)時的(de)(de)(de)(de)(de)光(guang)(guang)(guang)(guang)激(ji)發(fa)(fa)(fa)不可避(bi)免的(de)(de)(de)(de)(de)導(dao)致了光(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)背(bei)景信號(hao)，主要來自(zi)于激(ji)發(fa)(fa)(fa)光(guang)(guang)(guang)(guang)散(san)射和組織自(zi)發(fa)(fa)(fa)熒光(guang)(guang)(guang)(guang)、環(huan)境光(guang)(guang)(guang)(guang)，為了排除環(huan)境光(guang)(guang)(guang)(guang)、激(ji)發(fa)(fa)(fa)光(guang)(guang)(guang)(guang)散(san)射、自(zi)身熒光(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)背(bei)景信號(hao)影響，光(guang)(guang)(guang)(guang)電(dian)接(jie)(jie)收器(qi)前裝有濾光(guang)(guang)(guang)(guang)片，通過波(bo)長(chang)(chang)(chang)濾波(bo)能有效的(de)(de)(de)(de)(de)濾去燈珠自(zi)發(fa)(fa)(fa)光(guang)(guang)(guang)(guang)及部分自(zi)然光(guang)(guang)(guang)(guang)，接(jie)(jie)收熒光(guang)(guang)(guang)(guang)染(ran)料自(zi)身產生(sheng)的(de)(de)(de)(de)(de)熒光(guang)(guang)(guang)(guang)信號(hao)。熒光(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)近(jin)紅外(wai)波(bo)長(chang)(chang)(chang)是長(chang)(chang)(chang)于傳統的(de)(de)(de)(de)(de)肝功能檢測(ce)的(de)(de)(de)(de)(de)紫外(wai)光(guang)(guang)(guang)(guang)波(bo)長(chang)(chang)(chang)的(de)(de)(de)(de)(de)，自(zi)發(fa)(fa)(fa)光(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)底(di)噪也更(geng)低，在檢測(ce)原理上比依(yi)據(ju)朗伯-比爾定律的(de)(de)(de)(de)(de)紫外(wai)分光(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)度(du)計的(de)(de)(de)(de)(de)對(dui)比度(du)更(geng)高。信噪比的(de)(de)(de)(de)(de)定義為峰值信號(hao)減去背(bei)景信號(hao)的(de)(de)(de)(de)(de)差值，除以(yi)背(bei)景信號(hao)的(de)(de)(de)(de)(de)平(ping)方根，稱(cheng)之為sqrt（formulas?forcalculating?signal?to?noise?ratio?fsd?方法。

26、信(xin)噪比公(gong)式(shi)如下：

27、熒光(guang)光(guang)信號(hao)的(de)強(qiang)度存在(zai)疊(die)加效應，因此在(zai)測(ce)(ce)試時(shi)，未使用熒光(guang)劑時(shi)可(ke)以(yi)獲得(de)(de)背(bei)(bei)(bei)景(jing)信號(hao)，注射熒光(guang)劑后可(ke)以(yi)獲得(de)(de)測(ce)(ce)試信號(hao)，用測(ce)(ce)試信號(hao)減去(qu)背(bei)(bei)(bei)景(jing)信號(hao)可(ke)以(yi)得(de)(de)到(dao)優化后的(de)值(zhi)。本發明(ming)裝(zhuang)置光(guang)路(lu)設計在(zai)光(guang)電接(jie)收器上采取了(le)上通濾光(guang)片(pian)，能(neng)夠有效除去(qu)環境光(guang)及光(guang)源里的(de)背(bei)(bei)(bei)景(jing)信號(hao)，信噪(zao)(zao)比(bi)可(ke)達700，且室內自然光(guang)對背(bei)(bei)(bei)景(jing)信號(hao)無影響。光(guang)源信號(hao)與皮(pi)膚保持了(le)1～10mm的(de)距離，處(chu)于該位置信號(hao)強(qiang)度最強(qiang)，且能(neng)減少過于靠近光(guang)源時(shi)皮(pi)膚表層(ceng)組織(zhi)及背(bei)(bei)(bei)景(jing)的(de)漫(man)散射噪(zao)(zao)音，達到(dao)有效的(de)抗波動。

技術特征：

1.便攜熒(ying)光檢測(ce)器，其特征(zheng)在于：該檢測(ce)器包括供(gong)電電路、激發光源、采樣調理(li)、信(xin)號處理(li)、光電接收器、微處理(li)器、數(shu)據存儲電路；

2.根據權利(li)要求(qiu)1所述(shu)的(de)便攜熒(ying)光檢測器，其特征在于：所述(shu)供電(dian)電(dian)路(lu)部(bu)分由充(chong)電(dian)電(dian)路(lu)和(he)線性穩(wen)壓電(dian)路(lu)構成(cheng)；充(chong)電(dian)電(dian)路(lu)當轉換(huan)模塊插入時開(kai)始工(gong)作，對電(dian)池進行(xing)充(chong)電(dian)；線性穩(wen)壓電(dian)路(lu)將充(chong)電(dian)電(dian)池電(dian)壓轉成(cheng)穩(wen)定(ding)電(dian)壓輸出給(gei)后面負載設備(bei)供電(dian)。

3.根據權利要求2所述的便攜熒光(guang)檢測器，其特征在于(yu)：所述激發光(guang)源部分(fen)包(bao)括(kuo)開關控(kong)制(zhi)(zhi)電路(lu)和燈珠，光(guang)源波長范圍值優選為(wei)(wei)700nm～780nm；通過開關控(kong)制(zhi)(zhi)供電電路(lu)實現(xian)對燈珠持續性(xing)發光(guang)和間斷性(xing)發光(guang)兩(liang)種模式的切換；燈珠由(you)線(xian)性(xing)穩壓電路(lu)穩定供電，發射出(chu)波長范圍為(wei)(wei)630nm～780nm，中心(xin)波長為(wei)(wei)665nm，隨著距離(li)增加波長范圍至(zhi)597nm～992nm。

4.根(gen)據權利要求3所(suo)述的便攜熒光(guang)檢測(ce)器，其特征(zheng)在于(yu)：所(suo)述燈珠的范圍值優選為680～780nm。

5.根據權利要求(qiu)3所述(shu)的便(bian)攜熒光(guang)檢測器(qi)，其(qi)特(te)征在于：所述(shu)燈(deng)珠的范(fan)圍值優選為740～780nm。

6.根據權(quan)利要(yao)求1所述(shu)的便攜熒光(guang)檢測器，其特征(zheng)在于：所述(shu)裝置轉換器為ttl串usb模塊(kuai)，用于將電路板儲(chu)存的數據通過usb傳輸至(zhi)電腦。

7.根據權利(li)要求(qiu)1所述(shu)的(de)便攜(xie)熒(ying)光檢(jian)測器，其特(te)征在于：該檢(jian)測器的(de)使用步驟如下：

8.根據權(quan)利要求1所述(shu)的便攜熒光檢測器，其特征在(zai)于(yu)：icg色(se)素15分(fen)鐘滯留率(lv)的計算(suan)公式如(ru)下：

技術總結
本發明公開了便攜熒光檢測器，該檢測器包括供電電路、激發光源、采樣調理、信號處理、光電接收器、微處理器、數據存儲電路；所述激發光源與供電電路相連，而光電接收器與高精度uA級電流檢測電路相連；所述光電接收器用于接受穿過人體或者動物組織的熒光發射光并將其轉換成電信號；其中：所述采樣調理部分由可由優選為750nm、800nm、825nm，更優選為780nm，最優選為850nm的高通濾光片和高精度uA級電流檢測電路組成；本發明裝置體積小，續航時間長達48h～60h，電池可于使用中替換，便于隨身佩戴，檢測的同時不影響患者活動；發明裝置的存儲電路能儲存掉電時的數據，即使在斷電的情況下，也能將數據儲存到片外存儲，再次上電時仍能識別情況進行讀取操作。

技術研發人員：吳昊,徐徛,馬小斌,鄭寧洲,王心怡
受保護的技術使用者：陜西華耀新研生物科技有限公司
技術研發日：
技術公布日：2024/9/19