本(ben)實用新(xin)型涉及一種批量小鼠(shu)尾靜(jing)脈注射裝置,屬于實驗器材技術領域(yu)。
背景技術:
小(xiao)(xiao)(xiao)鼠(shu)(shu)是一(yi)種醫學(xue)研究(jiu)常用(yong)(yong)的(de)實(shi)驗動物(wu),對小(xiao)(xiao)(xiao)鼠(shu)(shu)進(jin)(jin)行(xing)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)高壓水動力注射來(lai)研究(jiu)基因(yin)表達或者建造模型,已經是一(yi)種廣泛用(yong)(yong)于(yu)科(ke)研的(de)成(cheng)熟實(shi)驗技(ji)術。尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)高壓水動力注射是指:在(zai)短時(shi)間內(6-8秒(miao))把含(han)大(da)量(liang)目的(de)基因(yin)質粒的(de)溶液通過(guo)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)快速注射到(dao)小(xiao)(xiao)(xiao)鼠(shu)(shu)體內。然(ran)而(er)小(xiao)(xiao)(xiao)鼠(shu)(shu)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)比(bi)較(jiao)纖(xian)細(xi),從小(xiao)(xiao)(xiao)鼠(shu)(shu)固(gu)定(ding)到(dao)其尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)充盈膨脹需要一(yi)定(ding)等待時(shi)間,當對大(da)批量(liang)小(xiao)(xiao)(xiao)鼠(shu)(shu)進(jin)(jin)行(xing)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)注射的(de)實(shi)驗操(cao)(cao)作時(shi),耗時(shi)就會更長,所以(yi)有效(xiao)地固(gu)定(ding)小(xiao)(xiao)(xiao)鼠(shu)(shu)和使其尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)充盈擴張能在(zai)很(hen)大(da)程度上降低實(shi)驗操(cao)(cao)作的(de)難度,節(jie)省實(shi)驗操(cao)(cao)作的(de)時(shi)間。目前(qian),適用(yong)(yong)于(yu)大(da)批量(liang)小(xiao)(xiao)(xiao)鼠(shu)(shu)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)注射的(de)固(gu)定(ding)裝(zhuang)置較(jiao)少,市場上用(yong)(yong)于(yu)單個小(xiao)(xiao)(xiao)鼠(shu)(shu)固(gu)定(ding)的(de)裝(zhuang)置多為透明半封閉裝(zhuang)置,因(yin)此當采用(yong)(yong)大(da)功(gong)率燈泡(pao)(pao)照射促使其尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)膨脹時(shi),由于(yu)溫度較(jiao)高,受熱不均,光照較(jiao)強(qiang),極易灼傷小(xiao)(xiao)(xiao)鼠(shu)(shu)眼睛。本裝(zhuang)置經過(guo)改進(jin)(jin),可避免在(zai)大(da)功(gong)率燈泡(pao)(pao)照射時(shi)灼傷小(xiao)(xiao)(xiao)鼠(shu)(shu)眼睛,同時(shi)可節(jie)約從固(gu)定(ding)小(xiao)(xiao)(xiao)鼠(shu)(shu)到(dao)其尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)充盈的(de)時(shi)間,在(zai)較(jiao)短時(shi)間內完成(cheng)大(da)批量(liang)小(xiao)(xiao)(xiao)鼠(shu)(shu)尾(wei)(wei)(wei)(wei)靜(jing)(jing)脈(mo)(mo)(mo)注射的(de)實(shi)驗操(cao)(cao)作。
技術實現要素:
本實用新(xin)型(xing)的目的是為了克(ke)服現有技術中的不足,從而提供(gong)一(yi)種(zhong)批量小鼠(shu)尾靜脈注(zhu)射裝置。
一(yi)種批量小(xiao)鼠尾靜脈注射裝(zhuang)置(zhi),包(bao)括T型(xing)支架(jia)(jia)、U型(xing)掛(gua)(gua)鉤、大(da)功率加熱燈、底座、圓(yuan)形實(shi)驗(yan)操作(zuo)臺(tai)(tai)、套(tao)管(guan)(guan)蓋、套(tao)管(guan)(guan),所述T型(xing)支架(jia)(jia)橫(heng)向桿(gan)兩端均設(she)(she)有U型(xing)掛(gua)(gua)鉤,兩個U型(xing)掛(gua)(gua)鉤均掛(gua)(gua)設(she)(she)有大(da)功率加熱燈;所述T型(xing)支架(jia)(jia)豎向桿(gan)中部設(she)(she)有底座,底座上設(she)(she)有圓(yuan)形實(shi)驗(yan)操作(zuo)臺(tai)(tai),圓(yuan)形操作(zuo)臺(tai)(tai)上設(she)(she)有套(tao)管(guan)(guan)蓋,套(tao)管(guan)(guan)蓋上設(she)(she)有套(tao)管(guan)(guan)。
所述(shu)套(tao)管(guan)上(shang)(shang)方(fang)設有通氣孔(kong),套(tao)管(guan)下部(bu)設有尾孔(kong),套(tao)管(guan)外壁(bi)尾孔(kong)上(shang)(shang)方(fang)設有U型環。
所述套(tao)管蓋通過螺絲釘固定在圓形操作臺上。
采用上述技(ji)術方案(an)的有益(yi)效果是:
本發明(ming)對(dui)現有的(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)裝置(zhi)進(jin)行(xing)了一(yi)定(ding)(ding)(ding)程度的(de)(de)(de)(de)(de)改(gai)造,第一(yi),把透(tou)明(ming)的(de)(de)(de)(de)(de)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)裝置(zhi)改(gai)為黑(hei)色(se)套管式固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)裝置(zhi),小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)除尾巴外(wai)全(quan)部固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)在(zai)黑(hei)色(se)套管中,在(zai)大功率加熱(re)燈泡(pao)的(de)(de)(de)(de)(de)照射下,黑(hei)色(se)套管使小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)盡量均(jun)勻受(shou)(shou)熱(re)后(hou)(hou)尾靜(jing)(jing)脈充盈(ying)彭胖,同時(shi)(shi)避免了燈泡(pao)的(de)(de)(de)(de)(de)強(qiang)光對(dui)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)眼睛的(de)(de)(de)(de)(de)灼傷。被(bei)扣在(zai)黑(hei)色(se)套管中的(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)由(you)于視線受(shou)(shou)阻,看不到(dao)周圍(wei)異(yi)常的(de)(de)(de)(de)(de)環(huan)境(jing)和實(shi)驗(yan)(yan)(yan)人員的(de)(de)(de)(de)(de)操(cao)作也會(hui)相對(dui)更(geng)加鎮靜(jing)(jing),便(bian)于實(shi)驗(yan)(yan)(yan)人員的(de)(de)(de)(de)(de)操(cao)作。第二,可固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)多(duo)只(zhi)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)(de)(de)圓形可旋轉實(shi)驗(yan)(yan)(yan)操(cao)作臺(tai)。此操(cao)作臺(tai)可同時(shi)(shi)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)多(duo)只(zhi)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu),當對(dui)A固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)位的(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)尾靜(jing)(jing)脈注(zhu)(zhu)射完畢時(shi)(shi),可把實(shi)驗(yan)(yan)(yan)操(cao)作完畢的(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)取出然后(hou)(hou)在(zai)A位置(zhi)重新(xin)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)另(ling)外(wai)一(yi)只(zhi)待操(cao)作小(xiao)(xiao)鼠(shu)(shu)(shu)(shu),固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)完畢后(hou)(hou)等(deng)待A小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)尾靜(jing)(jing)脈充盈(ying)的(de)(de)(de)(de)(de)時(shi)(shi)間(jian)可旋轉操(cao)作臺(tai)對(dui)B位置(zhi)已經(jing)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)且尾靜(jing)(jing)脈已經(jing)充盈(ying)的(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)進(jin)行(xing)實(shi)驗(yan)(yan)(yan)操(cao)作,節(jie)約(yue)了等(deng)待B位小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)從(cong)固(gu)(gu)(gu)(gu)(gu)(gu)(gu)定(ding)(ding)(ding)到(dao)待其尾靜(jing)(jing)脈充盈(ying)膨脹的(de)(de)(de)(de)(de)時(shi)(shi)間(jian),依次循環(huan)進(jin)行(xing)實(shi)驗(yan)(yan)(yan)操(cao)作 A B C D ……G H A B,此裝置(zhi)適用于一(yi)名(ming)或多(duo)名(ming)實(shi)驗(yan)(yan)(yan)人員同時(shi)(shi)進(jin)行(xing)實(shi)驗(yan)(yan)(yan)操(cao)作,可在(zai)較短時(shi)(shi)間(jian)內完成批量小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)(de)(de)尾靜(jing)(jing)脈注(zhu)(zhu)射實(shi)驗(yan)(yan)(yan)。
附圖說明
圖1為(wei)本實用新型批量(liang)小鼠(shu)尾靜脈注射裝置結構(gou)示意圖。
圖中:1-T型(xing)(xing)支架、2-U型(xing)(xing)掛鉤、3-大功率加熱燈、4-底座、5-圓形實(shi)驗(yan)操作臺、6-套管(guan)蓋、7-套管(guan)。
圖(tu)(tu)2為(wei)本實用新型批量(liang)小鼠尾靜脈注射裝置圖(tu)(tu)1中套(tao)管(guan)結構(gou)示意圖(tu)(tu)。
圖中:7-套管、8-通氣孔、9-尾孔、10-U型環。
圖3為(wei)本實用新型批量小(xiao)鼠尾(wei)靜脈注射裝置圖1中(zhong)套管蓋結構示意圖。
圖(tu)中:6-套管蓋、11-螺(luo)絲釘。
具體實施方式
一種批量小(xiao)(xiao)鼠尾(wei)靜(jing)脈注射裝(zhuang)置,如圖1所(suo)示:它包(bao)括(kuo)T型(xing)支(zhi)架1、U型(xing)掛(gua)(gua)鉤2、大功率(lv)加熱燈3、底座(zuo)(zuo)4、圓(yuan)形實(shi)驗操(cao)(cao)(cao)作(zuo)(zuo)(zuo)臺5、套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)蓋(gai)(gai)(gai)6、套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7,所(suo)述T型(xing)支(zhi)架1橫向(xiang)桿兩(liang)端均設(she)(she)有(you)U型(xing)掛(gua)(gua)鉤2,兩(liang)個U型(xing)掛(gua)(gua)鉤2均掛(gua)(gua)設(she)(she)有(you)大功率(lv)加熱燈3;所(suo)述T型(xing)支(zhi)架1豎向(xiang)桿中(zhong)部(bu)設(she)(she)有(you)底座(zuo)(zuo)4,底座(zuo)(zuo)4用(yong)于盛重(zhong)可旋轉(zhuan)的(de)(de)(de)圓(yuan)形實(shi)驗操(cao)(cao)(cao)作(zuo)(zuo)(zuo)臺5,圓(yuan)形操(cao)(cao)(cao)作(zuo)(zuo)(zuo)臺5上(shang)設(she)(she)有(you)套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)蓋(gai)(gai)(gai)6,套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)蓋(gai)(gai)(gai)6上(shang)設(she)(she)有(you)套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7;如圖2所(suo)示:所(suo)述套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7上(shang)方設(she)(she)有(you)通(tong)氣孔(kong)8,套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7下部(bu)設(she)(she)有(you)尾(wei)孔(kong)9,套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7外(wai)壁尾(wei)孔(kong)9上(shang)方設(she)(she)有(you)U型(xing)環10。套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7用(yong)于盛放小(xiao)(xiao)鼠,小(xiao)(xiao)鼠尾(wei)巴(ba)可從套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7側壁底端的(de)(de)(de)小(xiao)(xiao)鼠尾(wei)孔(kong)9處(chu)掏出,紗(sha)布條(tiao)可從套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7側壁的(de)(de)(de)U型(xing)環10處(chu)穿(chuan)過(guo),然后輕(qing)輕(qing)系住小(xiao)(xiao)鼠尾(wei)根部(bu),達到固定(ding)小(xiao)(xiao)鼠尾(wei)根部(bu)的(de)(de)(de)目的(de)(de)(de);如圖3所(suo)示:所(suo)述套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)蓋(gai)(gai)(gai)6通(tong)過(guo)螺(luo)絲釘11固定(ding)在(zai)圓(yuan)形操(cao)(cao)(cao)作(zuo)(zuo)(zuo)臺5上(shang)。把盛放小(xiao)(xiao)鼠的(de)(de)(de)套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)7通(tong)過(guo)旋轉(zhuan)和固定(ding)于圓(yuan)形實(shi)驗操(cao)(cao)(cao)作(zuo)(zuo)(zuo)臺5上(shang)的(de)(de)(de)套(tao)(tao)(tao)(tao)管(guan)(guan)(guan)蓋(gai)(gai)(gai)6相連(lian),即達到固定(ding)小(xiao)(xiao)鼠的(de)(de)(de)目的(de)(de)(de)。
本實用新型(xing)操(cao)作(zuo)方(fang)法(fa):
第一(yi)步:從套(tao)管7的(de)(de)底部尾(wei)孔9處將小(xiao)鼠尾(wei)巴拉出,然后用套(tao)管7側壁U型環10處穿出的(de)(de)紗布條將小(xiao)鼠尾(wei)根部輕輕系住(zhu),達到固定小(xiao)鼠尾(wei)根部的(de)(de)目的(de)(de),然后將套(tao)管7旋(xuan)轉固定在套(tao)管蓋6上,達到固定小(xiao)鼠的(de)(de)目的(de)(de);
第二步:按照同(tong)樣方法(fa)將(jiang)所有待實驗小鼠(shu)固定在實驗操作臺的不(bu)同(tong)位置,如A,B,C,D,E,F位等(deng);
第三步(bu):打開T型支(zhi)架(jia)1橫向桿兩端(duan)的大功率加熱燈3;
第(di)四(si)步:加熱(re)2-3分鐘后(hou),小(xiao)鼠(shu)(shu)(shu)尾靜脈(mo)充(chong)盈膨脹,即(ji)可進行(xing)尾靜脈(mo)注射(she)的(de)實驗操作(zuo);如從(cong)固(gu)定位(wei)(wei)A開始,當此處小(xiao)鼠(shu)(shu)(shu)操作(zuo)完(wan)畢(bi)后(hou),在(zai)此位(wei)(wei)重新(xin)固(gu)定另(ling)一只(zhi)待(dai)實驗小(xiao)鼠(shu)(shu)(shu),在(zai)固(gu)定位(wei)(wei)A處小(xiao)鼠(shu)(shu)(shu)加熱(re)的(de)時候,無(wu)須等待(dai),可旋轉的(de)圓形實驗操作(zuo)臺(tai)5,即(ji)刻(ke)進行(xing)固(gu)定位(wei)(wei)B處小(xiao)鼠(shu)(shu)(shu)的(de)尾靜脈(mo)注射(she),依(yi)次類推,循環(huan)進行(xing)實驗操作(zuo),此裝置(zhi)可在(zai)短(duan)時間內完(wan)成大批量小(xiao)鼠(shu)(shu)(shu)尾靜脈(mo)注射(she)的(de)實驗操作(zuo)。